Thanks @John Carbone but I don't think that my place fits the avatar of what you mentioned above where I would agree that 1 bathroom is less of an issue. I get mostly millennial and gen-x'ers, often traveling in pairs of couples, and very few kids. Plus I am not in a true "vacation" destination, and there's no room for an arcade anyway. I seem to get a lot of business travelers and people coming for concerts or weekend partying. I could see whoever is in the other bedroom (which is actually larger and has a fireplace; 40s construction lol) not being excited about trekking through the living room, kitchen and laundry room every time they need the bathroom. I am very clear about this in the listing and provide bathrobes, but this could be turning some potential guests away.
My thinking is that the 2nd bathroom would make it appealing to pairs of singles/couples who would each have their own master suite. Would also allow for a stronger MTR pivot or low-season strategy with the medical facilities (1 mile to a trauma level 2, 2.5 to a level 1), universities, and corporate headquarters in the area. Whether it would result in a noticeable revenue and occupancy is the mystery.