I would love to establish an LLC and then after closing transfer it to that. 

I have bid one one sfh, got it at a good price.I was to learn later that they transfer title/deed by way of a Quick Claim deed.

I have 125k of my 401k to transfer into a self directed IRA, 100k of which will be down payment and get a loan for the 220k.

I had many of the same Qs but my manager handled everything from the paperwork, transferring deposits etc. 

Once is transferred, you are responsible, make sure to have insurance quote ready and activate insurance as soon as it transfers to your  name

Personally, before the pandemic our firm had great success with live educational events that trained investors how to conduct appropriate due diligence on sponsors and their deals and we've transferred that to a new online platform that is aiming to do ostensibly do the same. 

Additionally I'd love to hear from some investors that built up over and time can look back and assess how what skills transferred from starting with single family versus making the leap to small commercial.

He has said he can add me to his will and make it irrevocable or do an irrevocable transfer of death deed for his home and a 25k stock portfolio. 

You can always form an LLC and transfer the property into the LLC AFTER escrow closes.

Your uncle will most likely have to get the loans in his name and then you can do a quit claim dead to transfer the title to the LLC.Doing business with family/friends can be difficult.